Composite

Part:BBa_K3924053:Design

Designed by: Yiyuan Huang   Group: iGEM21_Tsinghua   (2021-10-21)


ParaBAD-dCas9-linker-PmCDA1-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 311
    Illegal EcoRI site found at 1656
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 311
    Illegal EcoRI site found at 1656
    Illegal NheI site found at 300
    Illegal NheI site found at 1415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 311
    Illegal EcoRI site found at 1656
    Illegal BglII site found at 5091
    Illegal BamHI site found at 239
    Illegal BamHI site found at 3694
    Illegal XhoI site found at 4700
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 311
    Illegal EcoRI site found at 1656
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 311
    Illegal EcoRI site found at 1656
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5843
    Illegal SapI site found at 56


Design Notes

ParaBAD-dCas9-linker-PmCDA1-terminator


Source

ParaBAD-dCas9-linker-PmCDA1-terminator

References